Laying Out the Strategy for Analytical Method Selection for Charge Variants Across High DAR ADCs

  • Highlighting stability challenges with ADC icIEF analysis caused by cytotoxic payload hydrolysis at specific pH
  • Exploring how On-Board Mixing (OBM) enhances ADC sample stability with immediate sample mixing before analysis
  • Supporting potential use of Capillary Zone Electrophoresis as a backup method for charge variant analysis, especially where OBM is not feasible, to enhance the analytical toolkit